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KMID : 1161520180220030197
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Animal Cells and Systems
2018 Volume.22 No. 3 p.197 ~ p.204
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VIG-1 is required for maintenance of genome stability in Caenorhabditis elegans
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Vasamsetti Bala Murali Krishna
Park Yang-Seo
Cho Nam-Jeong
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Abstract
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To explore the function of VIG-1 in Caenorhabditis elegans, we analyzed the phenotypes of two vig-1 deletion mutants: vig-1(tm3383) and vig-1(ok2536). Both vig-1 mutants exhibited phenotypes associated with genome instability, such as a high incidence of males (Him) and increased embryonic lethality. These phenotypes became more evident in succeeding generations, implying that the germline of vig-1 accumulates DNA damage over generations. To examine whether vig-1 causes a defect in the DNA damage response, we treated worms with UV or camptothecin, a specific topoisomerase I inhibitor. We observed that the embryonic survival of the vig-1 mutants was reduced compared with that of the wild-type worms. Our results thus suggest that VIG-1 is required for maintaining genome stability in response to endogenous and exogenous genotoxic stresses.
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KEYWORD
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Caenorhabditis elegans, chromosome segregation, DNA damage response, genome stability, VIG-1
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